Label-free single-cell isolation of bacteria and other microorganisms, leverages brightfield imaging to isolate single bacteria from suspension samples.

DISPENSE BACTERIA, YEAST AND ARCHAEA USING SINGLE-CELL TECHNOLOGY

It is now possible to isolate individual prokaryotic cells using CYTENA’s validated single-cell dispensing technology. The B.SIGHT’s extremely high-resolution optics make even the smallest cells visible. No staining or labeling is required to isolate single bacterium.

Isolating cells is simple and contamination free with disposable cartridges. Simply pipette your sample into the reservoir and mount the cartridge to the B.SIGHT. Microscopic droplets are produced through our bacteria dispensing cartridges to create stable and accurate bacteria encapsulation.

IDEAL FOR ANAEROBIC CONDITIONS

The benchtop B.SIGHT can be operated in an anaerobic chamber thanks to its small footprint, low particle emission and low heat emission. These characteristics provide the ideal environment for isolating obligate anaerobes.

ISOLATE FLUORESCENT MICROORGANISMS

There is an option to add one integrated laser for fluorescent cell sorting.

Benefits:

  1. Fast and easy-to-use label-free isolation of single bacteria directly into liquid culture or agar
  2. Small benchtop instrument that integrates into fume hoods and anaerobic chambers
  3. Precise single-cell deposition into PCR well plates
  4. Sorting based on size, shape, and fluorescence
  5. Very high cell viability
  6. Assurance of clonality

Application areas:

  1. Synthetic biology
  2. Microbiome research
  3. Single-cell sequencing

List of pure cultures isolated with the B.SIGHTList of complex samples processed with the B.SIGHT
Bacillus subtilis
Bacillus vulgatus (strict anaerobe)
Enterococcus faecalis
Escherichia coli (Top10, DH5alpha, BL21, etc.)
Staphylococcus aureus
Various cyanobacteria strains
Saccharomyces cerevisiae (yeast)
Pichia pastoris (yeast)
Human faecal samples
Mouse caecal samples
Human oral microbiome
Wastewater
Marine samples
Aysia Khan
Application Engineer