The Nanolive holotomographic microscope allows for continuous and three-dimensional imaging of biological materials at a resolution exceeding the diffraction limits without any staining or sample preparation. The device provides three-dimensional tomographic imaging of the inside of cells with a resolution of 200 nm in the X and Y axes and 400 nm in the Z axis.

The device is equipped with a robotic arm that enables observation not only of the interior of a single cell, but also of the entire population.

Nanolive CX-A is used, among others, for the observation of stem cell differentiation, intercellular interactions (co-cultures) and host-pathogen interactions, observation of the immune response of cells and the analysis of mitochondrial dynamics. Due to the lack of phototoxicity of this technique (20 mW / cm2), it is an ideal tool for the continuous observation of cell interactions with hydrogel media.

Nanolive has a configured station to work with three epifluorescence channels, thanks to which we obtain morphological information derived from the holotomography technique and fluorescence information with one system.

Special EVE Analytics software can be added to the microscope, which thanks to the patented Artificial Intelligence algorithms allows for quantitative analysis of images obtained by means of holotomography.

Agata Jasińska
Agata Jasińska