The S.NEST, a high-throughput microbioreactor with CO2 incubator functions, shortens the process time for cell upscaling, provides a better microscale environment for cell growth, and brings more efficiency to cell line selection. It introduces suspension culture and late-stage bioreactor conditions to the early-stage cell line development pipeline, providing more growing space and oxygen than static cultures. When using the S.NEST, cells show higher density and viability compared to normal incubation, and weeks of cell expansion are no longer necessary.
S.NEST enables the simultaneous, high-throughput cultivation that enables the incubation of four 24-well or 96-well plates at once. Each of the plates is located in a separate chamber, which functions as an independent incubator with the possibility of controlling its temperature, CO2 level and humidity. Additionally, each chamber has a built-in UV lamp for sterilization.
The patented plate lids allow the continuous mixing of the suspension cultures in the wells and maintain an even distribution of the oxygen level. In order to reduce evaporation of the culture medium, a patented condenser integrated with the plate cover was used, which during each mixing cycle releases the liquid condensed on its surface back into the wells. By using optical sensors at the bottom of each well, it is possible to monitor the pH and oxygen saturation level of each well in real time. The entire process is controlled by intuitive, specialized software.